rabbit polyclonal antibody anti cb2  (Danaher Inc)

Journal: Acta histochemica

Article Title: Immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in the rat carotid body.

doi: 10.1016/j.acthis.2024.152205

Figure Lengend Snippet: Fig. 1. Immunohistochemistry for CB1 and CB2 in the carotid body. (A) Dot-like CB1 immunoreactivity is observed throughout the carotid body. Intense immu- noreactivity is shown in nerve fibers and/or endings (arrows). (B) A higher magnification view of the rectangle in panel A. Dot-like CB1 immunoreactivity is observed in the perinuclear cytoplasm of chemoreceptor cells (arrows). Intense immunoreactivity for CB1 is also present in nerve endings (arrowheads). (C) Dot-like CB2 immunoreactivity is noted throughout the carotid body. (D) A higher magnification view (rectangle in panel C) shows small dots of CB2 immunoreactivity in the perinuclear region of chemoreceptor cells (arrow). (E-G) Double immunofluorescence for CB1 and CB2 shows CB1-immunoreactive (arrows) and CB2- immunoreactive dots (arrow heads) in the same chemoreceptor cell; however, they are not colocalized.

Article Snippet: CB2: A rabbit polyclonal anti-CB2 antibody (NB300–606, Novus Biologicals, Centennial, CO, U.S.A.) was raised from a fusion protein that contained the first 33 amino acid residues of human CB2.

Techniques: Immunohistochemistry, Immunofluorescence

Journal: Acta histochemica

Article Title: Immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in the rat carotid body.

doi: 10.1016/j.acthis.2024.152205

Figure Lengend Snippet: Fig. 2. (A-C)Triple immunofluorescence for CB1 with TH and DBH. CB1-immunoreactive dot-like structures are observed in both TH- (arrows) and DBH- immunoreactive chemoreceptor cells (arrowheads). Dot-like immunoreactivity for CB1 localizes in both the perinuclear cytoplasm and outlines of the regions of immunoreactivity for TH or DBH. (D-F) Triple immunofluorescence for CB2 with TH and DBH. CB2 immunoreactivity is shown in the perinuclear cytoplasm of TH- (arrows) and DBH-immunoreactive chemoreceptor cells (arrowheads).

Article Snippet: CB2: A rabbit polyclonal anti-CB2 antibody (NB300–606, Novus Biologicals, Centennial, CO, U.S.A.) was raised from a fusion protein that contained the first 33 amino acid residues of human CB2.

Techniques: Immunofluorescence

Journal: Acta histochemica

Article Title: Immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in the rat carotid body.

doi: 10.1016/j.acthis.2024.152205

Figure Lengend Snippet: Fig. 3. (A-C) Double immunofluorescence for CB1 with P2X3. Intense CB1 immunoreactivity is surrounded by P2X3 immunoreactivity in the sensory nerve endings around chemoreceptor cells (arrows). (D-F) Double immunofluorescence for CB1 with VGluT2. CB1 immunoreactivity colocalizes with VGluT2 immunoreactivity, and appears to be in close contact with chemoreceptor cells (arrows). (G-H) Double immunofluorescence for CB2 with P2X2. Weak CB2-immunoreactive dots localize within P2X2-immunoreactive sensory nerve endings (arrows).

Article Snippet: CB2: A rabbit polyclonal anti-CB2 antibody (NB300–606, Novus Biologicals, Centennial, CO, U.S.A.) was raised from a fusion protein that contained the first 33 amino acid residues of human CB2.

Techniques: Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: Electroacupuncture reduces chronic itch via cannabinoid CB1 receptors in the ventrolateral periaqueductal gray

doi: 10.3389/fphar.2022.931600

Figure Lengend Snippet: Effect of EA on the expression of CB1 and CB2 receptor protein levels in the vlPAG. (A) The expression of c-Fos in the control group, AEW and EA group. (B) Representative gel image (upper) and quantification (bottom) of the protein level of CB1 receptors in control, AEW, EA and sham EA groups. (C) Representative gel image (upper) and quantification (bottom) of the protein level of CB2 receptors in control, AEW, EA and sham EA groups. GAPDH (36 kDa) was used as a loading control. The protein band at 60 kDa corresponds to the CB1 receptors protein. The protein band at 40 kDa corresponds to the CB2 receptors protein. Data are expressed as means ± SEM ( n = 6 mice in each group). * p < 0.05.

Article Snippet: Then, the transferred blot was blocked in Tris buffered saline (TBST) containing 5% skim milk powder at room temperature for 1 h. The membrane was placed in the following primary antibodies at 4°C overnight: rabbit anti-CB1 monoclonal antibody (1:1000, Cell Signaling Technology), rabbit anti-CB2 polyclonal antibody (1:500, Abcam), and mouse anti-GAPDH (0.5 μg/ml, Cloud-Clone Corp).

Techniques: Expressing, Control

Journal: Islets

Article Title: Cannabinoid receptors are differentially regulated in the pancreatic islets during the early development of metabolic syndrome.

doi: 10.1080/19382014.2020.1849927

Figure Lengend Snippet: Figure 2. Cannabinoid receptor expression in dissociated primary culture. Primary islet culture from control islets immunofluorescence showed the presence of cannabinoid in (A) for CB1 and (C) for CB2. The first column shows the merged image. Pancreatic β cells are identified by the insulin presence in red. The cannabinoid receptor is shown in green. For cellular identification, the DAPI-stained nucleus in blue is presented in the third column. Arrows indicate representative β cells, and non-β cells are indicated with arrowheads. Corrected fluorescence (see methods) for CB1 is presented in (B), and for CB2 in (D). Results are presented as Tukey box-plots with outliers and were compared with Mann-Whitney tests. Significant results are shown as ***p < .001.

Article Snippet: Primary antibodies were incubated overnight at 4°C as follows: CB1 rabbit polyclonal antibody 1:500 (Cayman Chemical Company, cat 10006590), CB2 rabbit polyclonal antibody 1:500 (Santa Cruz Biotechnology Inc., sc-25494), insulin goat polyclonal antibody 1:500 (Santa Cruz Biotechnology Inc., sc7839).

Techniques: Expressing, Control, Immunofluorescence, Staining, Fluorescence, MANN-WHITNEY